Optical biosensing of monkeypox virus using novel recombinant silica-binding proteins for site-directed antibody immobilization

Optical biosensing of monkeypox virus using novel recombinant silica-binding proteins for site-directed antibody immobilization

Blog Article

The efficient immobilization of capture antibodies is crucial for timely pathogen detection during global pandemic outbreaks.Therefore, we proposed a silica-binding protein featuring core functional domains (cSP).It comprises a peptide with a silica-binding tag designed to adhere to silica surfaces and tandem protein G fragments (2C2) for effective antibody capture.

This innovation facilitates precise site-directed immobilization of antibodies onto silica surfaces.We applied cSP to silica-coated optical fibers, creating a fiber-optic biolayer interferometer (FO-BLI) biosensor capable of monitoring the monkeypox virus (MPXV) protein A29L in spiked clinical samples to rapidly detect the MPXV.The cSP-based FO-BLI biosensor for MPXV demonstrated a limit Soup/Cereal Bowl of detection (LOD) of 0.

62 ng/mL in buffer, comparable to the 0.52 ng/mL LOD achieved using a conventional streptavidin (SA)-based FO-BLI biosensor.Furthermore, it achieved LODs of 0.

77 ng/mL in spiked serum and 0.80 ng/mL in spiked saliva, exhibiting no cross-reactivity with other viral antigens.The MPXV detection process was completed within 14 min.

We further proposed a cSP-based multi-virus biosensor strategy capable of detecting various pandemic strains, such as MPXV, the latest coronavirus disease (COVID) variants, and influenza A protein, to extend its versatility.The proposed cSP-modified FO-BLI biosensor has SHIRTS URBAN REGULAR FIT a high potential for rapidly and accurately detecting MPXV antigens, making valuable contributions to epidemiological studies.